In humans, CD36 deficiency is associated with reduced insulin signalling and hyperlipidaemia. In adipocytes and muscle cells, CD36 plays an important role in lipid homeostasis through the transport of long-chain fatty acids [19]. 2019 Oct 31;134(18):1484-1485. doi: 10.1182/blood.2019003022. MKs are identified by simultaneous labeling with LJ-P4 MoAb directed to the membrane CD41 (GPIIb/IIIa complex). Tomer A, Harker LA. While we cultured these leukemic cells in conditioned medlum prepared from phytohemagglutinin‐stimulated leukocytes and interleukin 3, expression of CD36(OKM5) antigen (thrombospondin receptor) increased gradually according to the differentiation and maturation of these cells. Ezetimibe also inhibits CD36-facilitated cholesterol uptake in COS-7 cells.56 It is still unclear whether the effects of CD36 on cholesterol uptake are direct. (B) Resolution of MKs by the expression of VWF. (D) Relationship between cell diameter, measured by time-of-flight, and ploidy of normal human marrow megakaryocytes (n = 19). Harker LA, Marzec UM, Kelly AB, et al.
Analysis of a cohort of 160 patients with thrombocytopenia and defined platelet life span, MMP-2, MMP-9, and TIMP-4 and Response to Aspirin in Diabetic and Nondiabetic Patients with Stable Coronary Artery Disease: A Pilot Study, Platelet distribution width for differential diagnosis of thrombocytosis, Megakaryocytes Found To Restrain Cell Migration In The Bone Marrow, Thrombocytopenia in the intensive care unit, Myeloid Neoplasm With Germline Predisposition: A 2016 Update for Pathologists, Use of Eltrombopag in Patients with Platelet Engraftment Failure Following Second Allogeneic Peripheral Stem Cell Transplantation, EFFECTS OF NUTRITION AND HYGIENE IN PATIENTS SUFFERING FROM ACUTE VIRAL FEVER WITHOUT/WITH LOW PLATELET COUNT, Shionogi Makes Mulpleta (Lusutrombopag) Available in US for the Treatment of Thrombocytopenia in Certain Patients, This mysterious enzyme can stop internal bleeding, megacystitis-microcolon-intestinal hypoperistalsis syndrome, Megakaryocyte Growth and Development Factor, megakaryocytic leukemia, megakaryocytic myelosis. (K) Figure shows the genes that are up-regulated in transcription from the brains of mice that have been infected with T. gondii for more than one year. Search for other works by this author on: Burstein SA, Harker LA. Our data showed that TSP-1 induced a dose-dependent growth inhibition in both murine and human colony forming unit-megakaryocyte (CFU-MK) assays and significantly counteracted the mitogenic effect from PEG-rHuMGDF.
Overexpression of CD36 in CHO cells facilitated AGE uptake [207]. For example, CD36 deficiency increases cellular efflux of cholesterol and phospholipids, effects that may be due at least in part to influencing membrane localization of the ATP cassette cholesterol transporter ABCA1.57. Data are mean ± SD. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The analysis of MK cellular differentiation markers is useful in studies investigating megakaryocytopoiesis in normal and disease states. 2B). CD36 was the first mammalian plasma membrane fatty acid transporter discovered (Abumrad et al., 1993). The expression of glycoprotein (GP)IIb/IIIa, GPIIIa, GPIb, and CD36 correlated directly with cell size and ploidy (r > 0.97); however, GPIb acquisition was relatively slow. Flow cytometric analysis of megakaryocyte ploidy: comparison with Feulgen microdensitometry and discovery that 8N is the predominant ploidy class in guinea pig and monkey marrow. N-TSP2-Fc significantly inhibited the growth and metastasis of breast tumors in a CD36-dependent manner (Koch et al., 2011). Aliquots of the marrow cell suspension were also incubated under identical conditions with isotype-matched monoclonal antibody (MoAb) and used as the control cell population. Figure 60.1. Preparation of bone marrow aspirates for flow cytometric analysis was performed as previously described.9-11 MKs were analyzed both in unfractionated marrow (to preclude possible selective cell loss) and in fractionated marrow (enriched for MKs to hasten the analysis). Eicke D, Baigger A, Schulze K, Latham SL, Halloin C, Zweigerdt R, Guzman CA, Blasczyk R, Figueiredo C. Sci Rep. 2018 Jul 5;8(1):10146. doi: 10.1038/s41598-018-28459-x. Jackson CW, Brown KL, Somerville BC, Lyles SA, Look AT. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. In: Rossi EC, ed. section 1734. Note that for clarity of presentation the schema does not accurately reflect the very large size of the TAG cargo relative to other PCTV components. Breton-Gorius J, Vainchenker W. Expression of platelet proteins during the in vitro and in vivo differentiation of megakaryocytes and morphological aspects of their maturation. Expression of myeloid markers by marrow MKs. Murray LJ, Bruno E, Uchida N, Hoffman R, Nayar R, Yeo EL, Schuh AC, Sutherland DR. Exp Hematol. © 2016 by The American Society of Hematology. Parks DR, Herzenberg LA. 16. NLM In platelets CD36 has been implicated in a large number of receptor functions including those for collagen, thrombospondin, oxidised LDL and for Plasmodium falciparum infected erythrocytes. | However, most of these studies focused on lipid metabolism, and owing to CD36/Cd36 promoter complexity, it is very likely that tissue-specific effects may influence gene expression regulation.
Tomer A, Harker LA, Burstein SA. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 U.S.C. CD36 can detect diacylglycerides and is thus. Bernard-Soulier syndrome: quantitative characterization of megakaryocytes and platelets by flow cytometric and platelet kinetic measurements. HHS
(I) Expression of ABCA4, VCAM1 and ICAM5 in hippocampal, perihippocampal and periventricular areas of infected mice (from Allen Atlas). Moreover, the growth suppression induced by TSP-1 was correlated with CD36 expression in megakaryocytic cell lines, where growth inhibition was demonstrated in CD36 positive (Meg-01, Dami and CHRF-288-11) but not in CD36 negative (M-07e) cell lines. Loken MR, Stall AM. An overlap in the emission spectra of the various fluorochromes was corrected by using single-color and double-color labeled cells of the same preparation. Enterocytes isolated from CD36-null mice exhibit reduced cholesterol uptake,22 and in vivo cholesterol output into the lymph is reduced by 50%.48 Other findings are also consistent with CD36 functioning in intestinal cholesterol absorption. In rodent gustatory cells, CD36 mediates fatty acid detection and preference for lipid ingestion [21,22], strongly suggesting a conserved role for this receptor in lipid sensorial recognition.
Relation between expression of GPIIb/IIIa, cell size, granularity, and ploidy of human marrow MKs. U54 DK106857/DK/NIDDK NIH HHS/United States, R01 DK094934/DK/NIDDK NIH HHS/United States, T32 DK007556/DK/NIDDK NIH HHS/United States, P30 CA016359/CA/NCI NIH HHS/United States, R01 DK086267/DK/NIDDK NIH HHS/United States, T32 HL007974/HL/NHLBI NIH HHS/United States. Latexin Inactivation Enhances Survival and Long-Term Engraftment of Hematopoietic Stem Cells and Expands the Entire Hematopoietic System in Mice. This glycoprotein complex (GPIIb–IIIa) binds plasma proteins, such as fibrinogen, fibronectin, von Willebrand factor, and vitronectin and plays a critical role in platelet aggregation. Epub 2018 Dec 19. Rosiglitazone, a proliferator-activated receptor [PPARγ] agonist, decreased inflammation and reduced parasitemia in a CD36 dependent manner in a P. chaubaudi hyperparasitaemia model. GPIs from P. falciparum activate TLRs and then activate MAPK and NFκB signalling pathways and CD36, thereby increasing cerebral pathology. Worthington RE, Nakeff A, Micko S. Flow cytometric analysis of megakaryocyte differentiation. We have shown that the expression of VWF is a more sensitive marker than GPIIb/IIIa for the identification of young marrow megakaryocytes. Similar to CD42, anti-CD41 and -CD61 monoclonal antibodies are frequently used for identification of megakaryocytic precursors in myeloproliferative disorders and myeloid leukemias. Coexpression of von Willebrand factor (VWF) and of GPIIb/IIIa by marrow MKs. For example, the assessment of receptor expression in myeloproliferative disorders may be of special interest because these disorders are invariably associated with platelet functional defects, leading to hemostatic compromise. Applying this assay to fluorescence-activated cell sorter-sorted cell populations, we found that the Lin(-)CD34(+)CD38(mid)CD45RA(-)FLT3(-)MPL(+)CD36(-)CD41(-) population is much more highly enriched for bipotent MEPs than any previously reported subpopulations. Kobayashi I, Kondo M, Yamamori S, Kobayashi-Sun J, Taniguchi M, Kanemaru K, Katakura F, Traver D. Sci Rep. 2019 Oct 2;9(1):14205. doi: 10.1038/s41598-019-50672-5. Cancer Metastasis Rev.